Journal: Cell Communication and Signaling : CCS
Article Title: Non-coding RNAs secreted by renal cancer include piR_004153 that promotes migration of mesenchymal stromal cells
doi: 10.1186/s12964-024-02001-1
Figure Lengend Snippet: tRNAs secreted by RCC cells. A. tRNAs altered in CM from Caki-1 and KIJ265T cell lines when compared with control RPTEC/TERT1 cells, classified by the transferred amino acid. The table shows results of RNAseq. B. Specification of tRNA isotypes with consistently altered expression in CM Caki-1 and CM KIJ265T (RNAseq data). C. qPCR validation of RNAseq results. Statistical analysis: One-way ANOVA with Dunnett’s multiple comparisons posttest. Data from 3-to-4 independent biological experiments
Article Snippet: The exosomal RNA samples were pretreated by demethylation followed by RNA precipitation and then reverse transcription was performed by using the rtStarTM tRNA - optimized First-Strand cDNA Synthesis Kit (Arraystar, Cat#: AS-FS-004, Rockville, MD, USA).
Techniques: Control, Expressing, Biomarker Discovery